Temperance Flowerdew, Lady Yeardley (1590 – 1628) was an early settler of the Jamestown Colony and a key member of the Flowerdew family, significant participants in the history of Jamestown. Temperance Flowerdew was wife of two Governors of Virginia, sister of another early colonist, aunt to a representative at the first General Assembly and "cousin-german" (first cousin) to the Secretary to the Colony.