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MAPseq or Multiplexed Analysis of Projections by Sequencing is a RNA-Seq based method for high-throughput mapping of neuronal projections. It was developed by Anthony M. Zador and his team at Cold Spring Harbor Laboratory and published in Neuron, a Cell Press magazine.

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rdfs:label	MAP-Seq (en)
rdfs:comment	MAPseq or Multiplexed Analysis of Projections by Sequencing is a RNA-Seq based method for high-throughput mapping of neuronal projections. It was developed by Anthony M. Zador and his team at Cold Spring Harbor Laboratory and published in Neuron, a Cell Press magazine. (en)
dcterms:subject	Molecular biology Molecular biology techniques RNA sequencing Neuroscience
Wikipage page ID	56977848 (xsd:integer)
Wikipage revision ID	1072664805 (xsd:integer)
Link from a Wikipage to another Wikipage	Messenger RNA Patch-sequencing Neuron Cold Spring Harbor Laboratory RNA-Seq Molecular biology Molecular biology techniques Quanta Magazine RNA RNA sequencing Neuroscience
Link from a Wikipage to an external page	https://www.quantamagazine.org/new-brain-maps-with-unmatched-detail-may-change-neuroscience-20180404%7Cwebsite=
sameAs	MAP-Seq MAP-Seq
dbp:wikiPageUsesTemplate	dbt:Cite_web dbt:Reflist dbt:Molecular-biology-stub dbt:Neuroscience-stub
has abstract	MAPseq or Multiplexed Analysis of Projections by Sequencing is a RNA-Seq based method for high-throughput mapping of neuronal projections. It was developed by Anthony M. Zador and his team at Cold Spring Harbor Laboratory and published in Neuron, a Cell Press magazine. The method works by uniquely labeling neurons in a source region by injecting a viral library encoding a diverse collection of RNA sequences ("barcodes"). The barcode mRNA is expressed at high levels and transported into the axon terminals at distal target projection regions. Following this, the cells from source and putative target regions of interest are harvested, and their RNA is extracted and sequenced. By matching the presence of the unique "barcode" in the source and target tissue, one can map the projections of neuron in a one-to-many fashion. (en)
prov:wasDerivedFrom	wikipedia-en:MAP-Seq?oldid=1072664805&ns=0
page length (characters) of wiki page	2162 (xsd:nonNegativeInteger)
foaf:isPrimaryTopicOf	wikipedia-en:MAP-Seq
is Link from a Wikipage to another Wikipage of	Patch-sequencing
is foaf:primaryTopic of	wikipedia-en:MAP-Seq

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